In vitro synthesis of different categories of specific protein by membrane-bound and free ribosomes.
نویسندگان
چکیده
The free and membrane-bound ribosomes of hepatic cells were isolated and used to program protein synthesis in cell-free extracts. Immuno-electrophoretic and radioautographic analysis of the products showed that the membrane-bound ribosomes synthesized serum proteins, whereas ribosomes free of endoplasmic reticulum synthesized specific nonserum liver proteins. After synthesis, the serum proteins remained associated with membrane vesicles, whereas the other liver proteins were found free of cytoplasmic organelles.
منابع مشابه
Protein synthesis by membrane-bound and free ribosomes of secretory and non-secretory tissues.
1. Methods for the separation of membrane-bound and free ribosomes from rat brain (cortex) and skeletal muscle were described and the preparations characterized by chemical analysis and electron microscopy. The attachment of ribosomes to membranes is not an artifact of the separation procedure. 2. The rate of incorporation of l-[(14)C]leucine into protein in vitro by the membrane-bound and free...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 63 4 شماره
صفحات -
تاریخ انتشار 1969